MICROSCÓPICOS AGUA DULCE

A veces observamos pequeños organismos cuando miramos muy cerca nuestro acuario, microbios, gusanos, etc. y algunos que haría falta una lupa o un microscopio para poder verlos. . . . . .

 

. . . . podríamos clasificar los organismos que pululan en el agua de nuestro acuario por su tamaño:

1 - Hasta 2 milimetros mesoplancton

 

2 - De 2 a 10 milimetros macroplancton

3 - Más de 10 milimetros megaloplancton

Entre 0 y 2 milimetros necesitariamos un microscopio para poder observarlos, algunos infusorios como los paramecios casi se pueden observar a simple vista o con una lupa.

 

Esta foto pertenece a: Nino Santamaria

El plancton se divide en:

HOLOPLACTON: es el plancton permanente, que a lo largo de todas sus etapas conserva un tamaño microscopico.

MEROPLACTON: es una etapa primaria en el desarrollo de algunas espacies, huevos y larvas de insectos, moluscos, anfibios, peces, etc.

Otra clasificación es el plancton vegetal (fitoplancton) y el plancton animal ( zooplancton)

 

 

Fotos Cristina Eisman - Pertenece a: regmurcia.com

Un acuario nuevo al principio esta carente de materia orgánica en disolución, pero con el tiempo y dependiendo de la temperatura, luz, y nutrientes ira floreciendo una colonia de seres vivos, normal y beneficiosa en el pequeño ecosistema que hemos creado, los rayos UVA, (lámparas germicidas), el cloro ó el ozono, y productos químicos como el permanganato de potasio reducen drásticamente la colonia beneficiosa de seres vivos microscópicos, por lo que utilizaremos estos medios de esterilización muy cuidadosamente o simplemente no los utilizaremos.

Algunos organismos los veremos porque están dentro del tamaño de macroplancton ó megaloplancton y los microscópicos no los veremos a simple vista, la mayoría de ellos son inocuos, e incluso beneficiosos para nuestro acuario, realizan funciones de filtración y clarificando el agua reduciendo elementos orgánicos que hay en disolución o en suspensión.

 

¿Como llegan estos organismos a nuestro acuario?

 

Pueden llegar a través del agua del grifo, sobre todo en zonas que no está muy tratada el agua potable, pero también pueden llegar en forma de esporas por el aire, los organismos enquistados y los huevos pueden resistir la congelación ó productos químicos desinfectantes muy fuertes, también a través de plantas importadas, en la piel o en el interior de los peces ó las heces de algún pez, de alguna forma el acuario es un ciclo cerrado agua y tarde o temprano pulularan muchos tipos de microbios, afortunadamente gracias a ello se completará el ciclo del nitrógeno y la vida no se acabará.

Habrá que distinguir de entre estos organismos los que son beneficiosos, y los que puedan causar enfermedades o parasitar a los peces ó plantas, gusanos de la piel, branquias, ácaros, hongos, bacterias, algas, etc. mientras los peces no den síntomas de malestar, irritaciones o movimientos convulsivos, pensaremos que esos bichitos hacen una función intermedia en la cadena de alimentación.

Prestaremos especial atención a las especies capturadas en la naturaleza sean peces o plantas, ó alimento vivo, tubifex, larvas de mosquito, dafnias, cyclops, etc. desechando lo que tengamos duda y sometiendo a observación, las plantas se deben desinfectar siempre, ó dejarlas un par de días en un acuario vacío de peces.

Los gusanillos que se desplazan por los vidrios son detritívoros, miden desde unos pocos milímetros a más de un cm. y finos como un hilo de coser, son inofensivos pero si tenemos demasiados es por el exceso de comida sobrante, algunos anabantidos los devoran, pueden ser planarias si tienen la cabeza en forma de flecha y estan sobre las plantas y cristales, y los alevines muy pequeños pueden ser atacados en su fase larvaria, para evitar su proliferación es conveniente hacer sifonados de grava de vez en cuando.

 

 

Esta foto pertenece a: ZOOINVERTEBRADOS

Muchos de los organismos microscópicos que no vemos, los infusorios por ejemplo que miden de 0.5 a 2 milímetros, de las que hay centenares de especies, paramecios, vorticelas, etc. son muy beneficios porque filtran el agua y sirven de alimento para los alevines más pequeños.

Las Hydras, son parecidas a las anemonas de mar pero mucho más pequeñas y solo peligrosas para los alevines recién nacidos, normalmente llegaran a nuestro acuario en alguna planta.

 

 

 

Esta foto pertenece a: microscopy-uk.org.uk

MICROCRUSTACEOS, hay más de 7000 especies de hasta un milímetro ó dos de longitud se alimentan de detritus, aumentados con un microscopio se parecen a artemias o gambas y son un excelente alimento para los alevines después de haber pasado la etapa de los infusorios, los alevines de discos se los pueden comer desde el principio.

 

 

Esta foto pertenece a: Invertebrados de agua dulce

Por último las microalgas flotantes de tono verde son el principal alimento del zooplancton, larvas de mosquito, gusanos, amebas, paramecios, etc.

 

 

Esta foto pertenece a: aquaticscape.com
 
 
 

Fuente :   El acuario y la electronica

 

 

 

Una guía sencilla para pequeña y microscópica vida de la charca con enlaces a recursos Micscape

Uno de los temas más gratificantes para el estudio con el microscopio son organismos de agua dulce. Métodos de recolección simples incluyen apretando las plantas de agua en una jarra y para especies libres de natación, una multa-Meshe
La forma más fácil de recoger los microorganismos y otra pequeña vida de la charca es exprimir el agua de las plantas de agua o espuma de la charca en un recipiente.

Otro método consiste en raspar el crecimiento de las plantas de agua u otras cosas que están cubiertos por un crecimiento verde o marrón . Una tarjeta de crédito de edad hará un excelente raspador pero tenga cuidado de no dejarlo caer en el estanque !

Se recomienda una red de plancton de las especies de natación libre ( planctónicas ) . Esta es una red hecha de una tela de malla muy fina con un pequeño recipiente en el extremo. También puede utilizar la red de plancton para concentrar el material expulsado de plantas de agua .
 
 
 
Las páginas de tablas y enlaces son una guía para algunos grupos comunes de organismos de agua dulce más pequeños (microscópicos de unos pocos milímetros de tamaño). Si no está familiarizado con un organismo, ver qué dibujo y características
El principiante también disfrutan de explorar la inmersión estanque virtual; haga clic en las criaturas en el tarro de aprender acerca de algunos de los organismos de agua dulce plebeyo.
 
 
Bacteri
 
unicelular , puntos o filamentos , apenas visible con un aumento más fuerte , las cianobacterias son más grandes
 
Introducción a las bacterias
Spirochaetes
 
unicelular, con diminutos pelos o pseudópodos
 
Ir a la visión general protozoos: por ejemplo, ciliados, ameba, heliozoos, euglenoids
 

 

Pond Life Identification Kit ~

A simple guide to small and microscopic pond life 
with links to Micscape resources

One of the most rewarding subjects for study with a microscope are freshwater organisms. Simple collecting methods include squeezing water plants into a jar and for free swimming species, a fine-meshed plankton net is recommended. For simple tips see how to collect microscopic pond life

The table and linked pages are a guide to some common groups of smaller freshwater organisms (microscopic to a few millimetres in size). If not familiar with an organism, see what drawing and features it most closely resembles in the table and then follow the links. 

The beginner may also like to explore the virtual pond dip; click on the creatures in the jar to learn about some of the commoner freshwater organisms.

Bacteria 

single celled, dots or strands, just visible with strongest magnification, cyanobacteria are larger 

Introduction to bacteria 
Spirochaetes

Protozoa 

single celled, with tiny hairs or pseudopodia 

 

Go to protozoa overview: e.g. ciliates, amoeba, heliozoa, euglenoids

Algae 

single celled, mostly green, sometimes yellow-brown 

 

Go to algae overview: eg. flagellates, diatoms, desmids, filamentous algae

Rotifers 

wheel-like, hairy appendages, transparent, free swimming or attached 0.2 - 1 mm

'Smallest page on the web' - rotifers

Gastrotrichs 

two tails, hairy, round mouth opening 
0.1 - 0.5 mm

No Micscape resources. (Articles welcomed!) 

Worms 

long thin body, many non related forms 

 

Go to worms overview: e.g. flatworms, annelids, nematodes

Bryozoa 

plant-like or jelly-like colony, crown of tentacles 
individuals: 0.25 - 5 mm

Bryozoans 
Pond fairies - Plumatella repens

Hydra 

green brown or colourless, body and tentacles contract and stretch  
extended: 20 mm

Introduction to hydra 
Hydra in 3D 
Hydra oligactis
Video clips of a hydra

Water bears 
(Tardigrades)

8 stumpy legs, slow moving 
<1 mm

See gallery links on the right for some of the finest video clips on the Web of these cute critters!

Hunting for 'bears' in the backyard 
The incredible water bear 
Water bear video gallery I 
Water bear video gallery II

Arthropods 

 

jointed limbs; many groups e.g. crustaceans ('water fleas'), mites 

 

Go to arthropods overview: e.g. ostracods, copepods, water fleas, mites etc.

other Arthropods: 
Insect stages

wide variety of forms

 

Go to insect stages overview: e.g. caddisfly larvae, dragonfly nymphs, water beetles, etc.

 

ProtozoaSome common freshwater types with links to Micscape resources

Protozoa are a very diverse group of organisms that vary widely in size, shape, features and habit. This page gives an overview of some commonly found freshwater protozoa.

The protozoa have been grouped by their major features. Some of these are artificial groups (i.e. not necessarily related to their taxonomy) but are convenient ones for the pond dipper. More about the classification of algae and protozoa.
Group

 

Key features 

Micscape links

Flagellates 
(those that photosynthesise are often classed as algae)

one or more flagella (whip-like cilia), phytoflagellates are green/ photosynthesise, zooflagellates are not green 
<0.4 mm

Flagellated protozoa - includes Euglena, Volvox

Some other common types: Monads e.g. Bodo, Choanoflagellates (flask-shaped with flared collar)

Amoeba

move with pseudopods 
0.02 - 5 mm

Amoeba - Protozoa portraits 
Amoeba - Video gallery 
Amoeba - Smallest page on the web 
Amoeba proteus - imaged by different illumination methods

Shelled amoeba

amoeba with a shell e.g. of sand grains 
0.1 - 0.4 mm

Protozoan houses - testate amoeba, Arcella, Nebela

Some other common types: Chaos, Pelomyxa

Heliozoans 
'Sun animalcules'

immobile, spherical with radiating hair-like pseudopods  
0.01 - 1 mm

Smallest page on the web - Heliozoans, Actinosphaerium 

Some other common types: Actinophrys, Acanthocystis

Ciliates - Peritrichs

cylindrical or bell-shaped bodies, undulating membrane of cilia, some stalked, often colonial and attached to animals or plants bell: <0.25mm 

Bell animalcules in 3D - Campanella 
Ophrydium - colonial, unstalked 
Vorticella - observations on its motile stage

Some other common types: Vorticella, Carchesium

Ciliates - Suctoria 

on water plants and other animals, adult ciliates have lost cilia, sticky tentacles capture prey <0.7 mm

Acineta - Suctoria, ciliates in disguise 
Podophyra - an interesting ciliate 
Some other common types: Tokophyra, Dendrocometes (lives on gill plates of f/water shrimp)

Other ciliates

 
Coleps 
 
Lacrymaria 
 
Paramecium 
 
Stentor 
 
Spirostomum

various, mostly free living forms

cell usually of a fixed shape but can be contractile, or extending neck, cilia of various forms, fixed mouth 
0.01 - 4 mm

Smallest page on the web - ciliates, Euplotes, Stylonichia, etc. 
Actinobolina vorax 
Amphileptus 
Coleps - a voracious protozoa 
Coleps & Urotricha - predation on rotiferColpidium - 
Protozoa portraits 
Colpoda - emerging from resting stage 
Didinium - a master feeder 
Dileptus - a carnivorous protozoa eating Litonotus 
Dileptus - notes on it eating Cyclidium 
Lacrymaria olor - the 'giraffe' of the protozoan world 
Lacrymaria olor - microscopic 'Loch Ness monster' 
Litonotus - being eaten by Dileptus 
Paramecium - an introduction 
Paramecium - Protozoa portraits 
Paramecium - by phase contrast 
How to study a 'pair of mecia' parts I-III- extensive articles on microscopy techniques to study paramecium's features. Ideal for students. 
Stentor - introduction 
Stentor - in 3D 
Spirostomum- one of the largest ciliates 
Spirostomum - observations on its feeding habits 
Tillina

Features of ciliates: 
Video gallery - beating cilia 
Cilia - an overview

 

 

 

 

 

Algae ~ Some common freshwater types with links to Micscape resources

Algae are a very diverse group of organisms that vary widely in size, shape, colour and habit. Ten or so phyla are represented in freshwater. This page gives an overview of some commonly found freshwater algae.

The algae have been grouped by their major features. Some of these are artificial groups (i.e. not necessarily related to their taxonomy) but are convenient ones for the pond dipper. More about the classification of algae and protozoa.

Group

 

Key features 

Micscape links

Flagellated forms (some are also often classed as protozoa) 
note: These flagella are hardly visible, only with strong magnification.

 

Euglenoids

green, flagella (whip-like cilia), free-swimming, red eye spot, body is flexible <0.4 mm

Flagellated algae/protozoa - includes Euglenaa heart shaped euglenoid: Phacus

Dinoflagellates

brown, 2 flagella, (1 in girdle), free-swimming, tough armour <0.4 mm

Peridinium - Stars of the Marshes

Green algae 
(Chlorophyta)

spherical colonies, cells with 2 flagella 
Volvox: 0.5 - 2mm

Volvox - the jewel of the pond 
Volvox in 3D 
Some other smaller colonial flagellates: Gonium, Eudorina, Pandorina,Sorastrum (rare), Synura, Uroglena

not all green algae are green

tiny, green/red, often in bird baths 
<0.05 mm

Haematococcus 
Haematococcus - image 
another small flagellated green algae: Chlamydomonas

There are many other small flagellated algae of non related groups. Classification is difficult.

Non-flagellated forms

 

Blue-green algae(cyanobacteria)

blue-green, often slow locomotion, 
used to be considered algae but more related to bacteria cells<0.05 mm colonies can be many mm

Smallest page on the web - 'bacteria' - image of a cyanobacteria

Diatoms

usually brownish, silica cell wall in two parts, solitary or colonial, some have a slow gliding motion 
<0.5 mm

Smallest page on the web - 'diatoms' 
Those who live in glass houses' 
Diatoms on strings 
Bacillaria - 'Carpenter's rule' diatom 
Library search - type diatom for articles on f/water & marine species 
Test diatoms - species to test optics

Desmids 
(Gamophyta: conjugating green algae)

green, no flagella, mainly solitary, some colonial, various shapes, two semi-cells which are mirror images 
<0.5 mm

What are desmids? 
Smallest page on the web - desmids 
Bill Ells' page - extensive articles on various species and aspects of desmids 
Desmids in 3D 
Desmid gallery

Green algae 
(Chlorophyta)

green, don't move, no flagella, not attached to a surface

starshaped colony: Pediastrum <0.3 mm 
bottom right: Scenedesmus <0.03 mm

Pediastrum - image only 
Pediastrum - the 'star' of the pond

Asterococcus 
Some other common 'non flagellates': Chlorella (symbiont in several organisms), Scenedesmus, Dictyosphaerium

Other algae of various growth forms

 

Water net

a sock-like colony, green algae (related to Pediastrum) up to 20 centimeters

Hydrodictyon - the water net

Some other common algae of various types: Botrydium, Chaetophora, Coelochaete, Enteromorpha

Filamentous forms

 

Pond scum 
(Gamophyta: conjugating green algae)

non-branching, green, chains of cells with distinctly shaped cell contents 
cell with <0.1 mm. length: centimeters

Spirogyra - chloroplasts like helix 
Spirogyra in 3D 
Zygnema - starshaped chloroplasts 
Mougeotia has plate-like chloroplasts

Other non-branchingforms

several non related groups

Oedogonium 
Some other common filamentous types: Tribonema, Ulothrix, Vaucheria

Branching forms

mostly green algae

Cladophora, Draparnaldia, Stigeoclonium

Red algae(Rhodophyta)

mainly marine, but some freshwater forms, not always red

Batrachospermum 
Porphyridium

 

Worms Some common freshwater types with links to Micscape resources

 

 

There are a number of groups of worms that occur in freshwater. Many aren't microscopic but are shown here to give an overview of these groups. Some common organisms that may be confused for a worm are also included.

Note: this table isn't comprehensive, there are several other freshwater groups like ribbonworms and horsehair worms.

Group

 

Key features

Micscape links

Flatworms
(Platyhelminthes)

flattened, 2 or more eye spots, move in gliding fashion
1 - 15+ mm

image of flatworm

Segmented worms
(Annelids)


oligochaetes: 1.5 mm. to >2cm long, hair bundles, (includes earthworm andTubifex)

leeches: characteristic 'leech-like' motion, suckers each end 
>1 cm

image of oligochaete

Roundworms
(Nematodes)

move very frantically, often in 'S' curves, 
0.2 - 10 mm

image of nematode

not to be mistaken for worms:

Hydra, has tentacles
extended: 2 cm

Introduction to hydra

larger ciliated protozoa like Spirostomum (has very fast contraction)

Spirostomum

insect stages like mosquito larva, these have a more distinct head than the worms above, also antennae/legs

Go to arthropods overview

 

 

 

 

 

 

 

Arthropods~ Some smaller freshwater types with links to Micscape resources

Arthropods are characterised by jointed limbs and include major groups like the crustacea, insects, spiders and mites. They have many segments, a tough outer skeleton and many modified limbs. There are many microscopic and macroscopic types that occur in freshwater.

Notes: 
There are several other groups with limb-like structures that aren't arthropods. Check rotifers and hydra on the first page.

Group

 

key features 

Micscape links

Crustacea - Ostracods

bean-like shell <2 mm

Freshwater crustacea

Crustacea - Copepods

long antennae, tiny eyespot: 0.5 - 3 mm

Freshwater crustacea 
Cyclops.The one-eyed pond organism

Crustacea - Water fleas 'Cladocera'

antennae, large compound eye 
0.3 - several mm

Water-flea anatomy - a fun interactive suite of images 
Water fleas - fascinating pond organisms 
The giant water flea Leptodora kindtii 
10 mm! 
Daphnia collecting techniques parts I-IV

Water bears (Tardigrades) 

8 stumpy legs body <1 mm

See gallery links on the right for some of the finest video clips on the Web of these cute critters!

Hunting for 'bears' in the backyard 
The incredible water bear 
Water bear video gallery I 
Water bear video gallery II

Water mites

8 legs, round body 0.5 - 5 mm

Water mites

Mosquito larvae (e.g. fly)

long body, often moves in S-shaped curves 1 - 20 mm

In praise of blackflies 
The mosquito Aedes aegypti and Dengue fever

Other Insect stages

wide variety of forms >1 mm

 

Go to Insect stages overview: e.g. caddisfly larva, dragonfly nymph, water beetles, etc.

 

Some larger freshwater crustacea -where Micscape has resources

Water louse (isopod) 10 mmFairy shrimp 10 cm and tadpole shrimp (branchiopods) 10 mm

Woodlouse or waterlouse. An interesting pond organismTadpole shrimp and fairy shrimp: Two rare British crustaceans

Freshwater shrimps(not true shrimps but amphipods)

curved, compressed body centimeter 
25 mm

No Micscape resources. (Articles welcomed!)

 

Protozoa

First Page 

Insects

Algae

Collecting tips 

Worms 

 

 

This page with links hopefully gives a useful overview, but it's neither a formal identification guide nor comprehensive. Water bears have been included here as well as on the first page, because some resources regard them as an arthropod phyla.

Acknowledgements: Many thanks to all the Micscape contributors whose articles this guide links to. For clarity their names are omitted in the links above. 


An Introduction to Microscopy

 

 

 

 

Insect stages Some larvae, nymphs and adult insects that live in freshwater

In pond water you will come across many insects, often perfectly adapted to the aquatic environment. Some species are entirely aquatic, whereas other insects only live in the water during their larval stages or as nymphs. When insects undergo a metamorphosis we call the immature form larva. When they gradually transform via moults into the adult form the young stages are called nymphs. This pages gives a simple overview of these stages and some of the adult forms. There are no Micscape links yet, articles welcomed!

Note on size: Many aquatic insects and their immature stages can vary in size from a few mm to 3cm or more depending on e.g. maturity and species, so sizes have been omitted for most groups. The shape and general features are a more reliable guide to a group than size.

Group

 

key features

Alderfly nymph

one tail, long filaments along the abdomen

Caddisfly larva

most species build a cylindrical case for protection, each species makes a distinct case from different material

Stonefly nymph

two jointed tails

Mayfly nymph

three jointed tails, leaf-like (or other shaped) 'gills' on its sides

Damselfly nymph

three leaf-like tail appendages (gills), bizarre extendable jaws

Dragonfly nymph

robust, no tail appendages, bizarre extendable jaws

Water bug
nymph/adult

no jaws, like all water bugs they possess a tube-like beak, the nymphs don't have wings,  
Some common forms: Backswimmer, water boatman. On the water surface: Pond skater

Water-beetle larva

strong jaws, long segmented body, short legs

Water beetle adult

strong jaws, tough shield, many water beetles are fierce predators

Springtail

the grey spring-tail (the most primitive insect group) Podura aquatica lives on the surface of the water, often in large numbers, 0.5-2.5mm
Visit the Postal Microscopical Society (UK) Springtail Group site for overview and projects

Mosquito larva

with a long slender body, often moves in S-shaped curves,

Dronefly larva

this so called rat-tailed maggot has a long tubed tail for breathing

 

Other Arthropodsthat are not insects


 

Go to arthropods overview: e.g. ostracods, copepods, water fleas, mites etc.

 

 

Protozoa

First Page

Arthropods

Algae

Collecting tips

Worms

 

 

This page with links hopefully gives a useful overview, but it's neither a formal identification guide nor comprehensive.

 


An Introduction to Microscopy

Comments to the compilers Wim van Egmond and Dave Walker are welcomed.

Microscopy UK Front Page
Micscape Magazine
Article Library

All images © Wim van Egmond

 

 


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